|
KMID : 0357919830170040432
|
|
Korean Journal of Pathology
1983 Volume.17 No. 4 p.432 ~ p.437
|
|
|
Effect of Thiol broth and Sodium Polyanethol Sulfonate on the Growth of Salmonella typhi Exposed to Antimicrobial Agents
|
|
이춘희/Chun Hee Lee
박숙자/정윤섭/이삼열/Sook Ja Park/Yun Sep Cho/Samuel Y. Lee
|
|
|
Abstract
|
|
|
Thiol broth and sodium polyanethol sulfonate(SPS) are reported to neutralize
antimicrobial agents to support better growth of bacteria in blood exposed to
antimicrobial agents. The effect has not been studied with S. typhi which is frequently
the object of blood culture in Korea. In this study the effect was determined in vitro
with artificial exposure of S. typhi to chloramhenicol, ampicillin, and cotrimoxazole. The
following results were obtained.
When chloramphenicol is present the growth of S. typhi Thiol broth was somewhat
worse than in Tryptic soy broth (TSB). In the presence of ampicillin or cotrimozasole,
the growth was better in Thiol broth than in TSB.
The growth was similar both in Thiol broth and Thiol broth＋SPS, and TSB and
TSB＋SPS, when chloramphenicol is present. The growth was slightly worse in the
Thiol broth＋SPS than in Thiol broth when 32 ㎍/㎖ of ampicillin is present; in Thiol
broth＋SPS than in Thiol broth when 32 ㎍/㎖ of cotrimoxazole is present; and in TSB
+ SPS than inTSB when 1 ㎍/㎖ of cotrimoxasole is present.
It is concluded that Thiol broth neutralities ampicillin and cotrimoxasole but not
chloramphenicol and the use of Thiol broth may be beneficial if used in actual blood
culture. SPS did not neutralize those antimicrobials.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
  
|
|
|
Listed journal information
|
|
  
|